Coding
Part:BBa_K4175012:Design
Designed by: Li Xianxiu, Zhang Wanying Group: iGEM22_ZJUintl-China (2022-09-25)
UAS-pSV40-CAR19-P2A-mCherry
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1388
Illegal NgoMIV site found at 1803 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 949
Design Notes
The sequence has been codon optimized for human.
Source
UAS-pSV40 (BBa_K4040033), CD8a signal peptide (BBa_K4040014), anti-CD19 (BBa_K2549005), CD8 hinge domain (BBa_K4175004), CD8 transmembrane domain (BBa_K4175005), 4-1BB (BBa_K4175006), CD3-ζ domain (BBa_K4175009)